Blood agar and chocolate agar are quite similar but they differ in preparation. Basal media are those that may be used for growth culture of bacteria that do not need enrichment of the media. These supplements are recommended for enriching gc agar media, proteose no. Agar m434 can also be used in place of chocolate agar base, which gives slightly better results than chocolate agar 4. Full text get a printable copy pdf file of the complete article 434k, or click on a page image below to browse page by page.
Blood agar consists of many ingredients but the primary ingredient is the blood, which came from a rabbit or a sheep. Chocolate agar ca is used for the selective cultivation and isolation of fastidious organisms. Colonies on chocolate agar are pinkishbrown and translucent, exhibit smooth consistency and defined margins, and are typically 0. Reactions incurred on chocolate agar by grampositive cocci were correlated with species identity. Detection of lipase and lecithinase activity of clostridium species. Chocolate agar is an enriched medium, thus nonpathogenic organisms may overgrow pathogenic bacteria. It will cause the agar to breakdown and become unusable. Bd chocolate agar gc ii agar with isovitalex and bd chocolate agar blood agar no. Chocolate agar is used for growing fastidious respiratory bacteria, such as haemophilus influenzae and neisseria meningitidis. This medium can be made selective by the addition of antibiotics. Colour chocolate brown coloured medium quantity of medium 25ml of medium in 90mm plate reaction 7. The cultivation medium for gonococci should ideally be a rich nutrients base with blood, either partially lysed or completely lysed. Yellowing of chocolate agar was associated with alphahemolytic species of streptococcus. Chocolate agar is prepared from molten blood agar base after adding 5 % defribinated sheep blood.
Sterility test passes release criteria expiry period when stored at performance and. Chocolate agar with tsa and growth supplements is a medium that supports the special growth requirements hemin and nad needed for the isolation of fastidious organisms, such as h. E39 should be used in parallel with this nonselective formula. Chocolate ii agar contains an improved gc agar base, bovine hemoglobin and isovitalex enrichment.
In december 2000, we published a letter pubmed pmid. Media blood agar plate bap medical laboratory technologist. Darkening and clearing of the medium was usually associated with the species staphylococcus epidermidis, staphylococcus aureus, staphylococcus simulans, and streptococcus faecalis. Description download pembuatan media chocolate agar comments. Media blood agar merupakan media pertumbuhan bakteri yang dapat membedakan bakteri pathogen berdasarkan efek exotoksin hemolitik bakteri pada sel darah merah. The study demonstrated that reactions occurring on chocolate agar are useful in identifying grampositive cocci. Chocolate eugon agar with 10% horse blood ceahb is a nonselective media to aid in the culturebased isolation and identification of a wide variety of fastidious pathogenic bacteria. Do not used media that shows signs of deterioration. It is treated and water and a number of other ingredients are added to sterilize the solution. In addition, if the specimen has been obtained from a neonate among whom e.
An enrichment medium should also be inoculated and one such as. The purpose is to inhibit the normal flora and nonpathogenic neisseria for the isolation of n. Chocolate agar is essentially the same as blood agar except that during preparation the red blood cells are lysed when added to molten agar. The medium is often used to observate the forms of haemolysis from pathogenic microorganisms. Nutrient agar, tryptic soy agar, potato dextrose agar, and standard methods agar plate count agar are appropriate alternatives together with an additional period 24 hours of incubation. Pdf suitability of a chocolate agarbased medium for the. Use water spray, dry chemical, carbon dioxide, or appropriate foam. Blood agar is an enriched, bacterial growth medium.
Thermo scientific chocolate agar medium chocolate agar. However, there have been more recent reports, including one from 1998 in which arvand et al. Blood agar, chocolate agar, and mycobacterium tuberculosis. Blood agar composition, preparation, uses vs chocolate. The name of the medium is derived from its chocolatebrown colored appearance which is obtained by the lysis of red blood cells. Chocolate agar culture media online microbiology notes. Bbl chocolate ii agar gc ii agar with hemoglobin and. The centrifuged deposit should be inoculated on chocolate agar and incubated in a humidified atmosphere and 10%co 2, and on blood agar incubated aerobically. The exceptions are blood agar, chocolate agar, kligler iron agar, lowensteinjensen agar, litmus milk broth, sim, and. Colony characteristics in chocolate agar neisseria gonorrhoeae. Chocolate agar, a differential medium for grampositive. The specimen to be cultivated should be streaked onto a chocolate agar plate or onto the agar slant. The name is itself derived from the fact that red blood cell rbc lysis gives the medium a chocolate brown color. Blood agar is a type of growth medium trypticase soya agar enriched with 5% sheep blood that encourages the growth of bacteria, such as streptococci, that otherwise wouldnt grow.
Chocolate agar choc or chocolate blood agar cba, is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. Thermo scientific prepared media biplate selection guide. Product principal combine collection, culture, result and microscopic observation in one ivd. Suatu media dikatakan media selektif apabila hanya ditumbuhi beberapa jenis mikroba sementara menghambat pertumbuhan mikroba jenis lain. The blood agar medium is prepared in a twostep process. Thermo scientific prepared media biplate selection guide for improved workflow efficiency across manual and automated methods. Summary and explanation chocolate agar was formulated in 1927 using peptones and yeast extract.
Chocolate agar base, with the addition of supplements, gives excellent growth of the gonococcus without overgrowth by contaminating organisms. Chocolate agar, a differential medium for grampositive cocci. First, a number of ingredients are added to water, including heart infusion brood, p. It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80c. Neisseriae sp hemophilus sp moraxella sp streak plate of ne eiisssseerriaa hggoonnoorrrrhooeaaee on chocolate agar. Whats the difference between chocolate agar medium and. Nacl a blood agar plate is put in boiling water for about 1 hour until its colour changes from red to chocolate brown. Chocolate agar is used for the isolation of fastidious organisms, such as haemophilus influenzae, when incubated at 3537c in a 5% co2 atmosphere. Chocolate agar media, is a nonselective, enriched growth medium used for the isolation of pathogenic bacteria like haemophilus influenza or neisseria spp. The name of the medium is derived from its chocolate brown colored appearance which is obtained by the lysis of red blood cells.
Chocolate agar media, is a nonselective, enriched growth medium used for the isolation of. Chocolate agar gc base with hemoglobin and supplements. Report pembuatan media chocolate agar please fill this form, we will try. Blood agar is an enriched medium in which nutritionally rich whole blood supplements the basic nutrients. Chocolate agar is used for growing fastidious respiratory bacteria, such as haemophilus.
This organism is oxidasepositive, and growth is best obtained on chocolate agar or thayer martin agar. Chocolate agar choc or chocolate blood agar cba is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. Thayermartin media is a chocolate agar supplemented with vancomycin, nystatin, and colistin to inhibit the normal flora, including nonpathogenic neisseria. Chocolate agar compositionbacteria culturedimportant.
The treatment and control of gonorrhea is complicated by increasing resistance to a variety of antimicrobial agents, including penicillin, tetracycline, and fluoroquinolones. Pdf neisseria gonorrhoeae isolates were grown on chocolate agar slants comprising gc agar medium base supplemented with 1% modified. Chocolate agar also contains red blood cells, but with chocolate agar, the blood is autoclaved, thus changing the red blood cells to a brown color. This culture medium can be used without blood for setting up blood cultures and as a base for preparing special culture media.
Chocolate agar is an enriched general purpose medium that supports the growth of most fastidious and nonfastidious organisms. Nutrient agar with mug 41 nutrient broth 41 of media 41 onpg rapid test broth 41 orange serum agar 41 oxford medium, modifi ed 41 pages saline 41 palcam 41 pc agar 41 phenylalanine agar slant 42 pea with 5% sheep blood 42 peptic digest agar with fildes. Chocolate agar contains less agar than other solid media therefore streaking should be done carefully to avoid gouging into the agar quality control after checking for correct ph, colour, depth, and sterility, the following organisms are used to determine the growth performance of the completed medium. Pdf pembuatan media chocolate agar free download pdf. Thayermartin media it is a chocolate agar supplemented with nystatin, vancomycin, and colistin. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. List of culture media used in microbiology with their uses. Solid media generally contain agar at a concentration of 1.
Chocolate agar manufacturer trade name chocolate agar product no. Most tube media both broth and agar can be stored at room temperature for about two months. Media blood agar bukan merupakan media selektif murni. Chocolate agar intended use remel chocolate agar is a solid medium recommended for use in qualitative procedures for the isolation and cultivation of fastidious organisms, especially neisseria and haemophilus species.
Haemophilus are small, pleomorphic, gramnegative coccobacilli. The organisms that can be isolated with chocolate agar include streptococcus pyogenes, haemophilus, francisella and neisseria. Chocolate agar plate medium enrichment type of medium required for the growth of certain fastidious organisms, including. Gc medium base supplement b supplement vx isovitalex. It is usually cultured on media that contain blood, but can also grow on a chocolate agar plate cap. Cap with bacitracin is a selective medium used to improve the primary isolation of h.
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